Casein Phosphopeptide-Amorphous Calcium Phosphate Reduces Streptococcus mutans Biofilm Development on Glass Ionomer Cement and Disrupts Established Biofilms
Stuart G Dashper, Deanne V Catmull, Sze-Wei Liu, Helen Myroforidis, Ilya Zalizniak, Joseph EA Palamara, N Laila Huq, Eric C Reynolds
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2016
Glass ionomer cements (GIC) are dental restorative materials that are suitable for modification to help prevent dental plaque (biofilm) formation. The aim of this study was to determine the effects of incorporating casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) into a GIC on the colonisation and establishment of Streptococcus mutans biofilms and the effects of aqueous CPP-ACP on established S mutans biofilms. S. mutans biofilms were either established in flow cells before a single ten min exposure to 1% w/v CPP-ACP treatment or cultured in static wells or flow cells with either GIC or GIC containing 3% w/w CPP-ACP as the substratum. The biofilms were then visualised using confoc..View full abstract
Awarded by Australian Government, Department of Industry, Innovation and Science
Supported by the Australian Government, Department of Industry, Innovation and Science. Grant ID 20080108. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.