Journal article
Genetic polymorphisms in the CD14 gene are associated with monocyte activation and carotid intima-media thickness in HIV-infected patients on antiretroviral therapy
YK Yong, EM Shankar, CLV Westhorpe, A Maisa, T Spelman, A Kamarulzaman, SM Crowe, SR Lewin
Medicine United States | LIPPINCOTT WILLIAMS & WILKINS | Published : 2016
Abstract
HIV-infected individuals on antiretroviral therapy (ART) are at increased risk of cardiovascular disease (CVD). Given the relationship between innate immune activation and CVD, we investigated the association of single-nucleotide polymorphisms (SNPs) in TLR4 and CD14 and carotid intima-media thickness (cIMT), a surrogate measurement for CVD, in HIV-infected individuals on ART and HIV-uninfected controls as a cross-sectional, case-control study. We quantified the frequency of monocyte subsets (CD14, CD16), markers of monocyte activation (CD38, HLA-DR), and endothelial adhesion (CCR2, CX3CR1, CD11b) by flow cytometry. Plasma levels of lipopolysaccharide, sCD163, sCD14, sCX3CL1, and sCCL2, were..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
SRL is supported by the Australian Centre for HIV and Hepatitis Research (ACH2) and by the National Health and Medical Research Council of Australia (NHMRC) through project grant 543137 and its Independent Research Institute Infrastructure Support Scheme. AK receives support from the Ministry of Higher Education, Malaysia, and University of Malaya High Impact Research Grant UM. C/625/1/HIR/MOHE/MED/01. EMS is supported by the University of Malaya Research Grant (UMRG) No. RG448-12HTM of the Health and Translational Medicine Research Cluster. AM is supported by a fellowship within the Postdoctoral Programme of the German Academic Exchange Service (DAAD) and by an Occupational Trainee Scholarship of the Burnet Institute. We gratefully acknowledge the Victorian Operational Infrastructure Support Program.