Journal article
Human glandular organoid formation in murine engineering chambers after collagenase digestion and flow cytometry isolation of normal human breast tissue single cells
CW Huo, D Huang, GL Chew, P Hill, A Vohora, WV Ingman, DJ Glynn, N Godde, MA Henderson, EW Thompson, KL Britt
Cell Biology International | WILEY | Published : 2016
DOI: 10.1002/cbin.10675
Abstract
Women with high mammographic density (MD) are at increased risk of breast cancer (BC) after adjustment for age and body mass index. We have developed a murine biochamber model in which both high MD (HMD) and low MD (LMD) tissue can be propagated. Here, we tested whether cells isolated by collagenase digestion and fluorescence-activated cell sorting (FACS) from normal breast can be reconstituted in our biochamber model, which would allow cell-specific manipulations to be tested. Fresh breast tissue was collected from women (n = 7) undergoing prophylactic mastectomy. The tissue underwent collagenase digestion overnight and, in some cases, additional FACS enrichment to obtain mature epithelial,..
View full abstractGrants
Awarded by Australian National Breast Cancer Foundation (NBCF) grant
Awarded by National Breast Cancer Foundation
Awarded by The Hospital Research Foundation
Funding Acknowledgements
We would like to thank St Vincent's BreastScreen, St Vincent's Hospital, Victoria for help with radiography and tissue sampling; Victoria Tissue Biobank and St Vincent's Department of Pathology for assistance with tissue accrual and processing; and the Experimental Medical and Surgical Research Unit, St Vincent's Hospital for mice-related experiment work. This work was supported in part by the Victorian Breast Cancer Research Consortium, the St. Vincent's Hospital Research Endowment Fund, the National Breast Cancer Foundation, and the University of Melbourne Research Grant Support Scheme (MRGSS). CWH was supported by the Australian Postgraduate Awards scholarship; KB was supported by an Australian National Breast Cancer Foundation (NBCF) Early Career Fellowship and Concept grant; GLC was supported by a postgraduate scholarship from the NHMRC; EWT was supported in part by the Australian National Breast Cancer Foundation (NBCF) grant (CG-10-04); NG was supported by and NBCF Innovator grant and a Peter MacCallum Foundation grant. This study benefited from support by the Victorian Government's Operational Infrastructure Support Program to St. Vincent's Institute.