Journal article
A BTP1 prophage gene present in invasive non-typhoidal Salmonella determines composition and length of the O-antigen of the lipopolysaccharide
E Kintz, MR Davies, DL Hammarlöf, R Canals, JCD Hinton, MW van der Woude
Molecular Microbiology | WILEY-BLACKWELL | Published : 2015
DOI: 10.1111/mmi.12933
Abstract
SalmonellaTyphimurium isolate D23580 represents a recently identified ST313 lineage of invasive non-typhoidal Salmonellae (iNTS). One of the differences between this lineage and other non-iNTS S.Typhimurium isolates is the presence of prophage BTP1. This prophage encodes a gtrC gene, implicated in O-antigen modification. GtrCBTP1 is essential for maintaining O-antigen length in isolate D23580, since a gtrBTP1 mutant yields a short O-antigen. This phenotype can be complemented by gtrCBTP1 or very closely related gtrC genes. The short O-antigen of the gtrBTP1 mutant was also compensated by deletion of the BTP1 phage tailspike gene in the D23580 chromosome. This tailspike protein has a putative..
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Awarded by Wellcome Trust
Funding Acknowledgements
This work was supported by the Wellcome Trust grant [WT094333MA]. We would like to thank Dr. Robert Heyderman for providing the D23580 strain, Dr. Robert Kingsley for providing the other iNTS isolates and Dr. Abram Aertsen for the gift of the wild-type P22 phage lysate. We would also like to thank Tom Taynton for generating the pMV333 vector and Sarah Broadbent for creating strain sMV523.