Chemical Synthesis of a Fluorescent IGF-II Analogue
Jade M Cottam, Denis B Scanlon, John A Karas, Antonio N Calabrese, Tara L Pukala, Briony E Forbes, John C Wallace, Andrew D Abell
International Journal of Peptide Research and Therapeutics | Springer | Published : 2013
Insulin-like growth factor II (IGF-II) is a protein with high structural and sequence similarity to insulin. Unlike insulin, it binds both the type 1 IGF receptor and the exon 11- isoform of the insulin receptor with high affinity. The overexpression and up regulation of IGF-II has been associated with the progression of various forms of cancer. The exact binding mechanism of IGF-II to its high affinity receptors is still not completely understood. Herein we describe the successful synthesis of a novel fluorescent IGF-II protein (F19Cou IGF-II), where residue 19 (phenylalanine) has been replaced by a fluorescent chromophore (coumaryl glycine). This novel coumaryl IGF-II analogue will be a us..View full abstract
The authors would like to acknowledge Clair Alvino for helpful discussions and assistance with competitive binding assays and the Australian Research Council (ARC) for funding.