Screening Strategies for TALEN-Mediated Gene Disruption
Boris Reljic, David A Stroud, H Puthalakath (ed.), CJ Hawkins (ed.)
PROGRAMMED CELL DEATH | HUMANA PRESS INC | Published : 2016
Targeted gene disruption has rapidly become the tool of choice for the analysis of gene and protein function in routinely cultured mammalian cells. Three main technologies capable of irreversibly disrupting gene-expression exist: zinc-finger nucleases, transcription activator-like effector nucleases (TALENs), and the CRISPR/Cas9 system. The desired outcome of the use of any of these technologies is targeted insertions and/or deletions (indels) that result in either a nonsense frame shift or splicing error that disrupts protein expression. Many excellent do-it-yourself systems for TALEN construct assembly are now available at low or no cost to academic researchers. However, for new users, scr..View full abstract
Awarded by Australian National Health and Medical Research Council (NHMRC)
We thank Mike Ryan, Michael Lazarou, Rochelle Tixeira, and Thanh N. Nguyen for reagents, advice, and discussions. This work was supported by the Australian National Health and Medical Research Council (NHMRC fellowship 1070916 to DAS) and the Australian Mitochondrial Disease Foundation (AMDF).