Development of a blocking ELISA for detection of Mycoplasma hyopneumoniae infection based on a monoclonal antibody against protein P65
Maojun Liu, Gaimei Du, Yue Zhang, Yuzi Wu, Haiyan Wang, Bin Li, Yun Bai, Zhixin Feng, Qiyan Xiong, Fangfang Bai, Glenn F Browning, Guoqing Shao
Journal of Veterinary Medical Science | JAPAN SOC VET SCI | Published : 2016
Mycoplasma hyopneumoniae causes porcine enzootic pneumonia, an economically important disease of swine. A more sensitive and reliable method for detection of serum antibodies is needed for epidemiological investigations and to evaluate the effect of immunization. We expressed the M. hyopneumoniae protein P65 in Escherichia coli and produced a monoclonal antibody (mAb) that bound specifically to recombinant P65. Using this mAb, a blocking enzyme linked immunosorbent assay (ELISA) was developed. The blocking ELISA had similar specificity to and sensitivity with the commercial ELISA produced by IDEXX. Thus, this blocking ELISA is a useful test for serological confirmation of M. hyopneumoniae in..View full abstract
Awarded by National Nature Science Foundation of China
Awarded by Jiangsu Agricultural Science and Technology Innovation Fund
This work was supported by the National Nature Science Foundation of China (31100136, 31370208 and 31300155) and the Jiangsu Agricultural Science and Technology Innovation Fund (CX (13) 3066).