Temporally controlled release of multiple growth factors from a self-assembling peptide hydrogel
Kiara F Bruggeman, Alexandra L Rodriguez, Clare L Parish, Richard J Williams, David R Nisbet
NANOTECHNOLOGY | IOP PUBLISHING LTD | Published : 2016
Protein growth factors have demonstrated great potential for tissue repair, but their inherent instability and large size prevents meaningful presentation to biologically protected nervous tissue. Here, we create a nanofibrous network from a self-assembling peptide (SAP) hydrogel to carry and stabilize the growth factors. We significantly reduced growth factor degradation to increase their lifespan by over 40 times. To control the temporal release profile we covalently attached polysaccharide chitosan molecules to the growth factor to increase its interactions with the hydrogel nanofibers and achieved a 4 h delay, demonstrating the potential of this method to provide temporally controlled gr..View full abstract
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Awarded by Australian Research Council Discovery Project
Awarded by NHMRC Career Development Fellowship
This research was supported by funding from the National Health and Medical Research Council, Australia and an Australian Research Council Discovery Project (DP130103131). K F Bruggeman was supported by a Natural Sciences and Engineering Research Council of Canada (NSERC) Postgraduate Scholarship Doctoral (PGS D) award. A L Rodriguez was supported by an Australian Postgraduate Award (APA). C L Parish was supported by a Senior Medical Research Fellowship provided by the Viertel Charitable Foundation, Australia. R J Williams was supported via an Alfred Deakin Research Fellowship. D R Nisbet was supported by a NHMRC Career Development Fellowship (APP1050684). Access to the facilities of the Centre for Advanced Microscopy (CAM) with funding through the Australian Microscopy and Microanalysis Research Facility (AMMRF) is gratefully acknowledged.