Dental plaque bacteria with reduced susceptibility to chlorhexidine are multidrug resistant
Hafiz Ghulam Murtaza Saleem, Christine Ann Seers, Anjum Nasim Sabri, Eric Charles Reynolds
BMC MICROBIOLOGY | BIOMED CENTRAL LTD | Published : 2016
BACKGROUND: Chlorhexidine (CHX) is used in oral care products to help control dental plaque. In this study dental plaque bacteria were grown on media containing 2 μg/ml chlorhexidine gluconate to screen for bacteria with reduced CHX susceptibility. The isolates were characterized by 16S rRNA gene sequencing and antibiotic resistance profiles were determined using the disc diffusion method. RESULTS: The isolates were variably resistant to multiple drugs including ampicillin, kanamycin, gentamicin and tetracycline. Two species, Chryseobacterium culicis and Chryseobacterium indologenes were able to grow planktonically and form biofilms in the presence of 32 μg/ml CHX. In the CHX and multidrug r..View full abstract
This work was supported by the Australian Government, Department of Industry, Innovation and Science and The Pakistan Higher Education Commission International Research Support Initiative Programme.