Hepatocyte glutathione peroxidase-1 deficiency improves hepatic glucose metabolism and decreases steatohepatitis in mice
Troy L Merry, Melanie Tran, Garron T Dodd, Salvatore P Mangiafico, Florian Wiede, Supreet Kaur, Catriona L McLean, Sofianos Andrikopoulos, Tony Tiganis
Diabetologia | SPRINGER | Published : 2016
AIMS/HYPOTHESIS: In obesity oxidative stress is thought to contribute to the development of insulin resistance, non-alcoholic fatty liver disease and the progression to non-alcoholic steatohepatitis. Our aim was to examine the precise contributions of hepatocyte-derived H2O2 to liver pathophysiology. METHODS: Glutathione peroxidase (GPX) 1 is an antioxidant enzyme that is abundant in the liver and converts H2O2 to water. We generated Gpx1 lox/lox mice to conditionally delete Gpx1 in hepatocytes (Alb-Cre;Gpx1 lox/lox) and characterised mice fed chow, high-fat or choline-deficient amino-acid-defined (CDAA) diets. RESULTS: Chow-fed Alb-Cre;Gpx1 lox/lox mice did not exhibit any alterations in bo..View full abstract
This work was supported by the National Health and Medical Research Council of Australia (TT and SA) and the Diabetes Australia Research Trust (TT).