Journal article
Anabolic action of parathyroid hormone (PTH) does not compromise bone matrix mineral composition or maturation
C Vrahnas, TA Pearson, AR Brunt, MR Forwood, KR Bambery, MJ Tobin, TJ Martin, NA Sims
Bone | ELSEVIER SCIENCE INC | Published : 2016
Abstract
Intermittent administration of parathyroid hormone (PTH) is used to stimulate bone formation in patients with osteoporosis. A reduction in the degree of matrix mineralisation has been reported during treatment, which may reflect either production of undermineralised matrix or a greater proportion of new matrix within the bone samples assessed. To explore these alternatives, high resolution synchrotron-based Fourier Transform Infrared Microspectroscopy (sFTIRM) coupled with calcein labelling was used in a region of non-remodelling cortical bone to determine bone composition during anabolic PTH treatment compared with region-matched samples from controls. 8 week old male C57BL/6 mice were trea..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
The authors gratefully acknowledge Mrs. Ingrid Poulton for expert technical assistance, Dr. Michael Gorman for advice on collagen structure, Dr. Eleftherios P Paschalis for help with spectral curvefitting, Drs Adele Boskey and Ljudmila Spevak for initial FTIR advice. This work was supported by National Health and Medical Research Council (Australia) Project Grant 1042129. N.A.S. is supported by a National Health and Medical Research Council (Australia) Senior Research Fellowship. St. Vincent's Institute is supported by the Victorian Government's Operational Infrastructure Support Programme. sETIRM data collection was undertaken on the infrared microspectroscopy (IRM) beamline at the Australian Synchrotron, Victoria, Australia.