Journal article
Transcriptomic effects of adenosine 2A receptor deletion in healthy and endotoxemic murine myocardium
KJ Ashton, ME Reichelt, SJ Mustafa, B Teng, C Ledent, LMD Delbridge, PA Hofmann, RR Morrison, JP Headrick
Purinergic Signalling | SPRINGER | Published : 2017
Abstract
Influences of adenosine 2A receptor (A2AR) activity on the cardiac transcriptome and genesis of endotoxemic myocarditis are unclear. We applied transcriptomic profiling (39 K Affymetrix arrays) to identify A2AR-sensitive molecules, revealed by receptor knockout (KO), in healthy and endotoxemic hearts. Baseline cardiac function was unaltered and only 37 A2AR-sensitive genes modified by A2AR KO (≥1.2-fold change, 4100 transcripts in wild-type (WT) myocardium (≥1.5-fold change, FDR < 1 %); the most induced are Lcn2 (+590); Saa3 (+516); Serpina3n (+122); Cxcl9 (+101) and Cxcl1 (+89) and the most repressed are Car3 (−38); Adipoq (−17); Atgrl1/Aplnr (−14); H19 (−11) and Itga8 (−8). Canonical respo..
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Awarded by National Heart, Lung, and Blood Institute
Funding Acknowledgements
This work was supported by the National Heart, Lung and Blood Institute grants HL-74001 (RRM), HL-027339 (SJM) and HL-48839 (PAH), an American Heart Association Southeast Affiliate Grant-in-Aid (PAH), a grant from the National Health and Medical Research Council of Australia (JPH) and the American Lebanese Syrian Associated Charities (RRM). We gratefully acknowledge the technical assistance of Kiel J. Headrick and Mary A. Cerniway.