Journal article

EFFECT OF HIGH-PROTEIN FEED SUPPLEMENTS ON CONCENTRATIONS OF GROWTH-HORMONE (GH), INSULIN-LIKE GROWTH FACTOR-I (IGF-I) AND IGF-BINDING PROTEIN-3 IN PLASMA AND ON THE AMOUNTS OF GH AND MESSENGER-RNA FOR GH IN THE PITUITARY-GLANDS OF ADULT RAMS

IJ CLARKE, TP FLETCHER, CC POMARES, JHG HOLMES, F DUNSHEA, GB THOMAS, AJ TILBROOK, PE WALTON, DB GALLOWAY

JOURNAL OF ENDOCRINOLOGY | J ENDOCRINOLOGY LTD | Published : 1993

Abstract

Three groups of mature rams were maintained on diets of hay, hay + 2% lupin or hay + 2% cowpea for 11 weeks. Serial blood samples were taken at 15-min intervals for 12 h for the determination of GH and IGF-I content by radioimmunoassay and for IGF-binding protein-3 (IGFBP-3) levels by Western blotting. The rams were killed after 77 days of supplementary feeding and their pituitary glands analysed for content of GH and GH mRNA. Mean plasma GH and baseline GH levels were significantly (P < 0.01) decreased in the rams fed lupin and cowpea compared with controls fed hay and GH pulse amplitude was significantly (P < 0.001) decreased in the group fed the cowpea diet. The frequency of GH pulses was..

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