Steady-state and time-resolved study of two-dimensional Förster energy transfer between 4-heptadecyl-7-hydroxycoumarin and RhB-DPPE in phospholipid air-water monolayers

Abstract

Förster energy transfer (FET) between 4-heptadecyl-7-hydroxycoumarin (HHC), the donor, and N-[[(lissamine rhodamine B)sulfonyl]dipalmitoyl]-L-α-phosphatidylethanolamine (RhB-DPPE), the acceptor, in dipalmitoyl-L-α-phosphatidylcholine (DPPC) and dioleoyl-L-α-phosphatidylcholine (DOPC) air-water monolayers was examined using both time-resolved and steady-state fluorescence methods. The existence of small molecular clusters of RhB-DPPE in DPPC can be readily identified by the FET technique, which then allows identification of component mixing/demixing regions in the compression of DPPC/HHC/RhB-DPPE monolayers. The time-resolved results from HHC showed complex fluorescence decay behavior due to ..