Journal article

The immunological response and post-treatment survival of DC-vaccinated melanoma patients are associated with increased Th1/Th17 and reduced Th3 cytokine responses

Claudia Duran-Aniotz, Gabriela Segal, Lorena Salazar, Cristian Pereda, Cristian Falcon, Fabian Tempio, Raquel Aguilera, Rodrigo Gonzalez, Claudio Perez, Andres Tittarelli, Diego Catalan, Bruno Nervi, Milton Larrondo, Flavio Salazar-Onfray, Mercedes N Lopez

CANCER IMMUNOLOGY IMMUNOTHERAPY | SPRINGER | Published : 2013

Abstract

INTRODUCTION: Immunization with autologous dendritic cells (DCs) loaded with a heat shock-conditioned allogeneic melanoma cell lysate caused lysate-specific delayed type hypersensitivity (DTH) reactions in a number of patients. These responses correlated with a threefold prolonged long-term survival of DTH(+) with respect to DTH(-) unresponsive patients. Herein, we investigated whether the immunological reactions associated with prolonged survival were related to dissimilar cellular and cytokine responses in blood. MATERIALS AND METHODS: Healthy donors and melanoma patient's lymphocytes obtained from blood before and after vaccinations and from DTH biopsies were analyzed for T cell populatio..

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Grants

Awarded by National Fund for Scientific and Technological Development


Awarded by Fund for the Promotion of Scientific and Technological Development


Awarded by Millennium Science Initiative from the Ministry of Economy, Development and Tourism


Funding Acknowledgements

Grants from the National Fund for Scientific and Technological Development (FONDECYT 1090243, 1090238, 3090044), the Fund for the Promotion of Scientific and Technological Development (FONDEF DO5I10366), and the Millennium Science Initiative from the Ministry supported this work for the Economy, Development and Tourism (P09/016-F).