Cell type-specific chromatin immunoprecipitation from multicellular complex samples using bits-chip

Abstract

This protocol describes the batch isolation of tissue-specific chromatin for immunoprecipitation (Bits-chIp) for analysis of histone modifications, transcription factor binding, or polymerase occupancy within the context of a multicellular organism or tissue. embryos expressing a cell type-specific nuclear marker are formaldehyde cross-linked and then subjected to dissociation. Fixed nuclei are isolated and sorted using Facson the basis of the cell type-specific nuclear marker. tissue-specific chromatin is extracted, sheared by sonication and used for chIp-seq or other analyses. the key advantages of this method are the covalent cross-linking before embryo dissociation, which preserves the t..