Journal article

THE EFFECTS OF LOW-DENSITY-LIPOPROTEIN AND HIGH-DENSITY-LIPOPROTEIN ON PHOSPHOINOSITIDE HYDROLYSIS IN BOVINE AORTIC ENDOTHELIAL-CELLS

DE MYERS, NH FIDGE, H STANTON, RG LARKINS

ATHEROSCLEROSIS | ELSEVIER SCI IRELAND LTD | Published : 1992

Abstract

Low density lipoprotein (LDL) and high density lipoprotein (HDL3) were tested for their ability to induce inositol phospholipid turnover and inositol phosphate production in bovine aortic endothelial cells (BAEC). The production of inositol phosphates following hydrolysis of the phosphoinositides was demonstrated by two methods; release of [3H]inositol phosphates after labelling with [3H]myo-inositol and by a direct binding assay for inositol 1,4,5-trisphosphate (InsP3). Acute exposure to LDL induced InsP3 release at low concentrations of the lipoprotein within the physiological range of LDL in tissues. HDL3 did not cause any release of the inositol phosphates. Pre-incubation of BAEC with HD..

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University of Melbourne Researchers