Journal article
Analysed cap mesenchyme track data from live imaging of mouse kidney development
JG Lefevre, AN Combes, MH Little, NA Hamilton
Data in Brief | ELSEVIER SCIENCE BV | Published : 2016
Abstract
This article provides detailed information on manually tracked cap mesenchyme cells from timelapse imaging of multiple ex vivo embryonic mouse kidneys. Cells were imaged for up to 18 h at 15 or 20 min intervals, and multiple cell divisions were tracked. Positional data is supplemented with a range of information including the relative location of the closest ureteric tip and a correction for drift due to bulk movement and tip growth. A subset of tracks were annotated to indicate the presence of processes attached to the ureteric epithelium. The calculations used for drift correction are described, as are the main methods used in the analysis of this data for the purpose of describing cap cel..
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Awarded by Australian Research Council
Funding Acknowledgements
This work was supported by Australian Research Council, Australia (DE150100652), National Health and Medical Research Council, Australia (APP1002748, APP1063696), and Human Frontiers in Science Program (RGP0039/2011). Microscopy was performed at the ACRF/IMB Cancer Biology Imaging Facility, which was established with the support of the Australian Cancer Research Foundation, and at the Murdoch Childrens Research Institute. A.N.C. holds a Discovery Early Career Researcher Award from the Australian Research Council. M.H.L. is a Senior Principal Research Fellow of the NHMRC. The authors declare no competing financial interests.