Intraocular pressure induced retinal changes identified using synchrotron infrared microscopy

HH Shen; GS Liu; SH Chow; JH Wang; Z He; C Nguyen; TW Lin; BV Bui

Journal article

Intraocular pressure induced retinal changes identified using synchrotron infrared microscopy

HH Shen, GS Liu, SH Chow, JH Wang, Z He, C Nguyen, TW Lin, BV Bui

Plos One | PUBLIC LIBRARY SCIENCE | Published : 2016

DOI: 10.1371/journal.pone.0164035

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Abstract

Infrared (IR) spectroscopy has been used to quantify chemical and structural characteristics of a wide range of materials including biological tissues. In this study, we examined spatial changes in the chemical characteristics of rat retina in response to intraocular pressure (IOP) elevation using synchrotron infrared microscopy (SIRM), a non-destructive imaging approach. IOP elevation was induced by placing a suture around the eye of anaesthetised rats. Retinal sections were collected onto transparent CaF2 slides 10 days following IOP elevation. Using combined SIRM spectra and chemical mapping approaches it was possible to quantify IOP induced changes in protein conformation and chemical di..

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University of Melbourne Researchers

Rick Liu Author

Christine Nguyen Author

Bang Bui Author

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Grants

Awarded by Australian Research Council

Funding Acknowledgements

This work was supported by the National Health and Medical Research Council of Australia (http://www.nhmrc.gov.au/) # 1106798 (HHS) #11081072 (GSL) #1061912 (JHW) NHMRC #1046203 (BVB) #1106798. BVB is supported by an Australian Research Council (www.arc.gov.au/) Future Fellowship (FT130100338). This work was also supported The Centre for Eye Research Australia receives Operational Infrastructure Support from the Victorian Government. (www.vic.gov.au/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.