Journal article

DNA methylation changes measured in pre-diagnostic peripheral blood samples are associated with smoking and lung cancer risk

Laura Baglietto, Erica Ponzi, Philip Haycock, Allison Hodge, Manuela Bianca Assumma, Chol-Hee Jung, Jessica Chung, Francesca Fasanelli, Florence Guida, Gianluca Campanella, Marc Chadeau-Hyam, Kjell Grankvist, Mikael Johansson, Ugo Ala, Paolo Provero, Ee Ming Wong, Jihoon Joo, Dallas R English, Nabila Kazmi, Eiliv Lund Show all

INTERNATIONAL JOURNAL OF CANCER | WILEY | Published : 2017

Abstract

DNA methylation changes are associated with cigarette smoking. We used the Illumina Infinium HumanMethylation450 array to determine whether methylation in DNA from pre-diagnostic, peripheral blood samples is associated with lung cancer risk. We used a case-control study nested within the EPIC-Italy cohort and a study within the MCCS cohort as discovery sets (a total of 552 case-control pairs). We validated the top signals in 429 case-control pairs from another 3 studies. We identified six CpGs for which hypomethylation was associated with lung cancer risk: cg05575921 in the AHRR gene (p-valuepooled  = 4 × 10-17 ), cg03636183 in the F2RL3 gene (p-valuepooled  = 2 × 10 - 13 ), cg21566642 and c..

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Grants

Awarded by NHMRC


Awarded by ERC


Awarded by CRUK


Awarded by German Center for Lung Research (DZL)


Awarded by Cancer Research UK


Awarded by Medical Research Council


Funding Acknowledgements

Grant sponsor: NHMRC; Grant numbers: 209057, 251553, 1050198; Grant sponsor: ERC; Grant number: ERC-2008-AdG-232997; Grant sponsor: CRUK; Grant number: C18281/A19169; Grant sponsor: German Center for Lung Research (DZL); Grant number: PB13394; Grant sponsors: VicHealth and Cancer Council, Victoria; Roy Castle Lung Cancer Foundation the MRC Integrative Epidemiology Unit; European Commission (SANCO); the German Cancer Aid (Deutsche Krebshilfe); German Federal Ministry of Education and Research (BMBF)