Journal article
Distinctive expression of interleukin-23 receptor subunits on human Th17 and γδ T cells
BD Wines, ML Yap, MS Powell, PS Tan, KK Ko, E Orlowski, P Mark Hogarth
Immunology and Cell Biology | WILEY | Published : 2017
DOI: 10.1038/icb.2016.93
Abstract
The interleukin-23 (IL-23) pathway, T helper 17 (Th17) cells and γδ T cells, which respond to IL-23, have major pro-inflammatory roles. We have used unique IL-23 receptor (IL-23R) subunit-specific monoclonal antibodies, X67 and X68, and IL-12 receptor beta-1 subunit (IL-12Rβ1) expression levels to evaluate the IL-23R complex on CD4 β TCR Th17 cells and on γδ T cells. Both IL-23R and IL-12Rβ1 subunits constitute the functional IL-23R. Expression of the IL-23R subunit by cultured Th17 cells was heterogeneous. Th17 cells expressed consistent high levels of the IL-12Rβ1 subunit, which appeared a better predictor of responsiveness to IL-23 than the expression of the IL-23R subunit. Moreover, sort..
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Funding Acknowledgements
We thank Andy Coley, Amanda Gavin and Doreen Krumbiegel for helpful discussions and Noel Warner for suggesting combining the anti-IL-23R antibodies. We thank Jeanne LeMasurier (AMREP Flow Cytometry Core Facility) for flow sorting, Soong Ling and Alicia Chenoweth for expert technical assistance and Halina Trist for reading the manuscript. This work was funded by the National Health and Research Council project grants and the Cooperative Research Centre for Biomarker Translation and the Victorian Operational Infrastructure Scheme. KK was supported by Arthritis Australia.