Functionally distinct roles for different miR-155 expression levels through contrasting effects on gene expression, in acute myeloid leukaemia
N Narayan, L Morenos, B Phipson, SN Willis, G Brumatti, S Eggers, N Lalaoui, LM Brown, HJ Kosasih, RC Bartolo, L Zhou, D Catchpoole, R Saffery, A Oshlack, GJ Goodall, PG Ekert
Leukemia | NATURE PUBLISHING GROUP | Published : 2017
We thank the staff at WEHI Bioservices (Crystal Stivala, Nicole Lynch, Julie Merryfull and Jessica Mansheim) for assistance with animal work; the staff at WEHI Flow Facility for assistance with FACS sorting; Dr Francoise Mechinaud (Head of Oncology, Royal Children's Hospital), Dr Ngaire Elwood (Cord blood bank, MCRI), Dr Nick Wong (MCRI), Minhee Halemba (MCRI) and Jane Ng (MCRI) for procurement and preparation of paediatric samples. Grants from the Leukaemia Foundation (Australia), the National Health and Medical Research (NHMRC) Project grant, the Victorian Cancer Agency, My Room and the Children's Cancer Centre Foundation supported paediatric AML work. We thank the Children's Cancer Centre Tissue Bank (MCRI, The Royal Children's Hospital) for supplying tumour samples and coded data, which is funded through generous support by CIKA (Cancer In Kids @ RCH), Leukaemia Auxiliary at RCH, MCRI and the Royal Children's Hospital. We also thank Aedan Roberts and Aysen Yuksel for sample preparation at the Tumour Bank at the Children's Hospital at Westmead, which is generously supported by The Kids Cancer Project. We are greatful to Dr Johannes Zuber (The Research Institute of Molecular Pathology, Austria) for MYB and KIT shRNAs and Drs Mark McKenzie (WEHI), Luisa Cimmino (NYU School of Medicine, New York) and Ross Dickins (Monash University) for PU.1 shRNAs. We thank Andrew Bert (SA Pathology, Australia) for valuable technical expertise. Paul G Ekert is supported by the Children's Cancer Foundation and the Steven Walter Children's Cancer Foundation. Nisha Narayan was supported by the Australian Postgraduate Award PhD scholarship (University of Melbourne).