CRISPR/Cas9 Targets Chicken Embryonic Somatic Cells In Vitro and In Vivo and generates Phenotypic Abnormalities
Kwaku Dad Abu-Bonsrah, Dongcheng Zhang, Donald F Newgreen
Scientific Reports | NATURE PUBLISHING GROUP | Published : 2016
Awarded by NHMRC
We thank Damien Hudson (MCRI) and Craig Smith (MCRI) for the DT40 and DF-1 cells respectively, Marguerite Evans-Galea (MCRI) for the mouse brain and Yoshiko Takahashi (Kyoto, Japan) for Tol2 and transposase plasmids. We thank Matthew Burton and Paul Lau (Flow Cytometry-MCRI), Paul Kalitsis (Chromosomal Research-MCRI), Hwee Ong (Genetic Health Research-MCRI) and Alex Combes (Kidney Development, Disease and Regeneration) for their technical advice and support. The mCherry monoclonal antibody (3A11) was developed and supplied by the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242. This research was supported by NHMRC grant 1069757 and MCRI. HSCR-related constructs were contributed to by Foundation for Children 2014-211. We acknowledge the Victorian Government's Operational Infrastructure Support Program to MCRI. K.D.A.-B. holds an IPRS and APA(Int) PhD Scholarship through the Department of Paediatrics, University of Melbourne.