Journal article
A desferrioxamine B squaramide ester for the incorporation of zirconium-89 into antibodies
SE Rudd, P Roselt, C Cullinane, RJ Hicks, PS Donnelly
Chemical Communications | ROYAL SOC CHEMISTRY | Published : 2016
DOI: 10.1039/c6cc05961a
Abstract
A squaramide ester derivative of desferrioxamine B (H3DFO) that is compatible with aqueous solvents and does not induce antibody aggregation is used to attach the siderophore to the antibody trastuzumab. The new conjugates were radiolabeled with zirconium-89 to give complexes that are more resistant to ligand exchange when compared to currently used H3DFO derivatives and result in high quality positron emission tomography images in mouse models of HER2 positive breast cancer.
Grants
Funding Acknowledgements
We acknowledge: the ARC and NHMRC for financial support, Professor Roger I. Price for provision of <SUP>89</SUP>Zr, Rachael Walker, Kerry Ardley and Susan Jackson for technical assistance.