Characterization of Sarcoptes scabiei cofilin gene and assessment of recombinant cofilin protein as an antigen in indirect-ELISA for diagnosis
Yu Zheng, Ran He, Manli He, Xiaobin Gu, Tao Wang, Weimin Lai, Xuerong Peng, Guangyou Yang
BMC Infectious Diseases | BMC | Published : 2016
BACKGROUND: Scabies impairs the health of humans and animals and causes heavy economic losses. Traditional diagnostic methods for scabies are inefficient and ineffective, and so far there is no commercial immunodiagnostic or molecular based test for scabies. METHODS: Here, we used recombinant Sarcoptes scabiei cofilin protein as an antigen to establish indirect ELISA. S. scabiei cofilin is highly homologous to Dermatophagoides farinae Der f 31 allergen (90% identity). The S. scabiei cofilin gene was cloned and expressed in Escherichia coli to obtain recombinant protein. Western blotting and fluorescence immunohistochemistry were carried out, and we established an indirect ELISA method and de..View full abstract
Awarded by Research Fund for the Chengdu Research of Giant Panda Breeding
This study was supported by a grant from the Research Fund (Project No. CPF2014-17) for the Chengdu Research of Giant Panda Breeding.