Cloning and characterization of a novel sigma-like glutathione S-transferase from the giant panda parasitic nematode, Baylisascaris schroederi
Yue Xie, Xuan Zhou, Lin Chen, Zhihe Zhang, Chengdong Wang, Xiaobin Gu, Tao Wang, Xuerong Peng, Guangyou Yang
Parasites and Vectors | BMC | Published : 2015
BACKGROUND: Baylisascaris schroederi, an intestinal nematode of the giant panda, is the cause of the often fatal disease, baylisascariasis. Glutathione S-transferases (GSTs) are versatile enzymes that can affect parasite survival and parasite-host interactions and, are therefore, potential targets for the development of diagnostic tests and vaccines. METHODS: In this study, we identified a full-length cDNA that encoded a novel, secretory sigma-like GST (Bsc-GSTσ) from a B. schroederi-omic dataset. Following cloning and sequencing, sequence and structural analyses and comparative modeling were performed using online-bioinformatics and proteomics tools. The recombinant Bsc-GSTσ (rBsc-GSTσ) pro..View full abstract
Awarded by Research Fund for the Chengdu Giant Panda Breeding
This work was supported by the grant from the Research Fund for the Chengdu Giant Panda Breeding (No. CPF-2012-13). We would like to thank Mei Liu and Yu Wang (College of Veterinary Medicine, Sichuan Agricultural University, China) for technical assistance; Caiwu Li (Ya'an Bifengxia Research Base of Giant Panda Breeding, China) for materials.