Ligand-Dependent Modulation of G Protein Conformation Alters Drug Efficacy
Sebastian George Barton Furness, Yi-Lynn Liang, Cameron James Nowell, Michelle Louise Halls, Peter John Wookey, Emma Dal Maso, Asuka Inoue, Arthur Christopoulos, Denise Wootten, Patrick Michael Sexton
Cell | CELL PRESS | Published : 2016
Awarded by National Health and Medical Research Council (NHMRC)
Awarded by NHMRC Program Grant
This work was funded by National Health and Medical Research Council (NHMRC) Project Grant APP1061044 and NHMRC Program Grant APP1055134. P.M.S. is an NHMRC Principal Research Fellow and A.C. an NHMRC Senior Principal Research Fellow. M.L.H. is a NHMRC RD Wright Fellow (1061687). D.W. is an NHMRC Career Development Fellow. A. I. was funded by JST, PRESTO. We thank N.A. Lambert for the constructs encoding G<INF>alpha s</INF><SUP>72</SUP>:RLuc8 and G<INF>alpha s</INF><SUP>72</SUP>:mCherry and for critiquing the manuscript. We thank B.K. Kobilka for the construct encoding Nb35 and for advice on expression and purification of the CTR ternary complex from insect cells. We thank P. Zhao and J.R. Lane for discussions. We also thank J.B. Furness and M.L. Whitelaw for critiquing the manuscript.