Journal article
Serrulatane Diterpenoid from Eremophila neglecta Exhibits Bacterial Biofilm Dispersion and Inhibits Release of Pro-inflammatory Cytokines from Activated Macrophages
HH Mon, SN Christo, CP Ndi, M Jasieniak, H Rickard, JD Hayball, HJ Griesser, SJ Semple
Journal of Natural Products | AMER CHEMICAL SOC | Published : 2015
Abstract
The purpose of this study was to assess the biofilm-removing efficacy and inflammatory activity of a serrulatane diterpenoid, 8-hydroxyserrulat-14-en-19-oic acid (1), isolated from the Australian medicinal plant Eremophila neglecta. Biofilm breakup activity of compound 1 on established Staphylococcus epidermidis and Staphylococcus aureus biofilms was compared to the antiseptic chlorhexidine and antibiotic levofloxacin. In a time-course study, 1 was deposited onto polypropylene mesh to mimic a wound dressing and tested for biofilm removal. The ex-vivo cytotoxicity and effect on lipopolysaccharide-induced pro-inflammatory cytokine release were studied in mouse primary bone-marrow-derived macro..
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Awarded by Australian Government
Funding Acknowledgements
This work was partially supported by the Australian Government under the Co-operative Research Centres Program-The Wound Management Innovation CRC Grant Number 2.12 to H.J.G., S.J.S., J.D.H., and H.R. The University of South Australia provided a Ph.D. scholarship to H.H.M. We acknowledge Adelaide Microscopy at the University of Adelaide, an AMMRF facility for the microscopy. We thank Andrew Beck for photography of the plates.