Journal article

Individual and population quantitative analyses of calcium flux in T-cells activated on functionalized material surfaces

SN Christo, GT Sarvestani, SS Griesser, BR Coad, HJ Griesser, K Vasilev, MP Brown, KR Diener, JD Hayball

Australian Journal of Chemistry | Published : 2012

Abstract

We have developed a novel method for activating T-cells on material surfaces that enable individual and population-based analyses of intracellular calcium flux, as a quantitative measure of T-cell receptor engagement. Functionalized material surfaces were created using a plasma-polymerized foundation layer to immobilize stimulatory T-cell ligands, which could induce T-cell receptor-dependent calcium flux in naive T-cells. Real-time confocal microscopic detection and quantification of calcium flux using paired fluorescent ratiometric probes facilitated the tracking and analysis of response profiles of individual T-cells, as well as population analyses using a combination of individual T-cell ..

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University of Melbourne Researchers

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