Journal article
A novel ligand of calcitonin receptor reveals a potential new sensor that modulates programmed cell death
S Furness, D Hare, A Kourakis, A Turnley, P Wookey
Cell Death Discovery | NATURE PUBLISHING GROUP | Published : 2016
Abstract
We have discovered that the accumulation of an anti-calcitonin receptor (anti-CTR) antibody conjugated to a fluorophore (mAb2C4:AF568) provides a robust signal for cells undergoing apoptotic programmed cell death (PCD). PCD is an absolute requirement for normal development of metazoan organisms. PCD is a hallmark of common diseases such as cardiovascular disease and tissue rejection in graft versus host pathologies, and chemotherapeutics work by increasing PCD. This robust signal or high fluorescent events were verified by confocal microscopy and flow cytometry in several cell lines and a primary culture in which PCD had been induced. In Jurkat cells, GBM-L2 and MG63 cells, the percentage un..
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Funding Acknowledgements
SGBF was partly funded by Program Grant 1055134 and Project Grant 1061044 from the National Health and Medical Research Council (NHMRC) of Australia. We thank Sandy Fung (Drug Discovery Biology Laboratory, Monash Institute of Pharmaceutical Science, Monash University) for the FACS analysis and Mr Ben O'Shea for generating the data in Supplementary Figure S1a, lanes 8-10. We thank Professor Terry Johns (Monash Institute of Medical Research, Clayton, Australia) for provision of the cell line GBM-L2 and Professor Mike Ryan (Monash University) for making available the cell lines HCT116 and HCT116 (bak<SUP>-/-</SUP>bax<SUP>-/-</SUP>). Confocal microscopy was performed at the Biological Optical Microscopy Platform, The University of Melbourne (www.microscopy.unimelb.edu.au).Thanks go to the team at the Antibody Facility (Walter & Eliza Hall Institute, Bundoora) for making the anti-CTR antibodies.