Journal article
Small interfering RNA-mediated suppression of Ccl2 in Muller cells attenuates microglial recruitment and photoreceptor death following retinal degeneration
Matt Rutar, Riccardo Natoli, Jan M Provis
JOURNAL OF NEUROINFLAMMATION | BMC | Published : 2012
Abstract
BACKGROUND: The recruitment and activation of inflammatory cells is thought to exacerbate photoreceptor death in retinal degenerative conditions such as age-related macular degeneration (AMD). We investigated the role of Müller cell-derived chemokine (C-C motif) ligand (Ccl)2 expression on monocyte/microglia infiltration and photoreceptor death in light-mediated retinal degeneration, using targeted small interfering (si)RNA. METHODS: Adult Sprague-Dawley rats were injected intravitreally with 1 μg of either Ccl2 siRNA or scrambled siRNA, and were then exposed to 1000 lux of light for a period of 24 hours. The mice were given an overdose of barbiturate, and the retinas harvested and evaluated..
View full abstractGrants
Awarded by Australian Research Council Centres of Excellence Program Grant
Funding Acknowledgements
This study was supported by an Australian Research Council Centres of Excellence Program Grant (CF0561903),