Practical Lessons in Murine Thoracic Lymph Duct Cannulations: Observations in Female and Male Mice Across Four Different Strains That Impact on "Cannulatability"
Suzanne M Caliph, David M Shackleford, David B Ascher, Lisa M Kaminskas
JOURNAL OF PHARMACEUTICAL SCIENCES | WILEY-BLACKWELL | Published : 2015
Cannulation of the thoracic lymph duct in experimental animals allows direct measurement of the lymphatic exposure of lymph-targeted drugs. When coupled with recent advances in genetically modified and diseased mouse models, this presents further opportunities to define changes in biological processes and disease in response to drug treatment. Although cannulation of the thoracic lymph duct in mice is inherently challenging because of the small size and delicate nature of the duct, it can be further confounded by anatomical variations between animals. In this communication, we present our observations on the anatomical features of the thoracic lymph duct between mice of different strains and..View full abstract
Awarded by NHMRC
L.M.K. was supported by an NHMRC Career Development fellowship (APP1022732). D.B.A. was supported by a NHMRC CJ Martin fellowship (APP1072476). This work was supported by an Australian NHMRC grant.