Journal article
Mycobacterium tuberculosis whole genome sequencing and protein structure modelling provides insights into anti-tuberculosis drug resistance
J Phelan, F Coll, R McNerney, DB Ascher, DEV Pires, N Furnham, N Coeck, GA Hill-Cawthorne, MB Nair, K Mallard, A Ramsay, S Campino, ML Hibberd, A Pain, L Rigouts, TG Clark
BMC Medicine | BIOMED CENTRAL LTD | Published : 2016
Abstract
Background: Combating the spread of drug resistant tuberculosis is a global health priority. Whole genome association studies are being applied to identify genetic determinants of resistance to anti-tuberculosis drugs. Protein structure and interaction modelling are used to understand the functional effects of putative mutations and provide insight into the molecular mechanisms leading to resistance. Methods: To investigate the potential utility of these approaches, we analysed the genomes of 144 Mycobacterium tuberculosis clinical isolates from The Special Programme for Research and Training in Tropical Diseases (TDR) collection sourced from 20 countries in four continents. A genome-wide ap..
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Awarded by King Abdullah University of Science and Technology
Funding Acknowledgements
JP is supported by a BBSRC PhD studentship. The project was supported by the KAUST faculty baseline research fund (KAUST-BRF) to AP. The authors wish to thank members of KAUST Bioscience Core laboratory who sequenced the isolate DNA. DBA is supported by an NHMRC CJ Martin Fellowship (APP1072476). DEVP is supported by Rene Rachou Research Center (CPqRR/FIOCRUZ Minas). DBA and DEVP are funded by a Newton Fund RCUK-CONFAP Grant awarded by The Medical Research Council (MRC) and Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG). NF is funded by a Medical Research Council Methodology Research Fellowship (MR//K020420). TGC is funded by the Medical Research Council UK (Grant no. MR/K000551/1, MR/M01360X/1, MR/N010469/1). There are no conflicts of interests.