Journal article

mCSM-AB: a web server for predicting antibody-antigen affinity changes upon mutation with graph-based signatures

Douglas EV Pires, David B Ascher

NUCLEIC ACIDS RESEARCH | OXFORD UNIV PRESS | Published : 2016

Abstract

Computational methods have traditionally struggled to predict the effect of mutations in antibody-antigen complexes on binding affinity. This has limited their usefulness during antibody engineering and development, and their ability to predict biologically relevant escape mutations. Here we present mCSM-AB, a user-friendly web server for accurately predicting antibody-antigen affinity changes upon mutation which relies on graph-based signatures. We show that mCSM-AB performs better than comparable methods that have been previously used for antibody engineering. mCSM-AB web server is available at http://structure.bioc.cam.ac.uk/mcsm_ab.

Grants

Awarded by Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG)


Awarded by NHMRC


Awarded by Medical Research Council


Funding Acknowledgements

Newton Fund RCUK-CONFAP Grant awarded by The Medical Research Council (MRC) and Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG) (MR/M026302/1 to D.E.V.P., D.B.A.); Rene Rachou Research Center (CPqRR/FIOCRUZ Minas), Brazil (to D.E.V.P.); NHMRC CJ Martin Fellowship [APP1072476 to D.B.A.]. Funding for open access charge: MRC.