Journal article

Isolation of conditional mutations in genes essential for viability of Cryptococcus neoformans

Giuseppe Ianiri, Kylie J Boyce, Alexander Idnurm

Current Genetics | SPRINGER | Published : 2017

Abstract

Discovering the genes underlying fundamental processes that enable cells to live and reproduce is a technical challenge, because loss of gene function in mutants results in organisms that cannot survive. This study describes a forward genetics method to identify essential genes in fungi, based on the propensity for Agrobacterium tumefaciens to insert T-DNA molecules into the promoters or 5' untranslated regions of genes and by placing a conditional promoter within the T-DNA. Insertions of the promoter of the GAL7 gene were made in the human pathogen Cryptococcus neoformans. Nine strains of 960 T-DNA insertional mutants screened grew on media containing galactose, but had impaired growth on m..

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University of Melbourne Researchers