Activity and conformation of lysozyme in molecular solvents, protic ionic liquids (PILs) and salt-water systems
Emmy C Wijaya, Frances Separovic, Calum J Drummond, Tamar L Greaves
PHYSICAL CHEMISTRY CHEMICAL PHYSICS | ROYAL SOC CHEMISTRY | Published : 2016
Improving protein stabilisation is important for the further development of many applications in the pharmaceutical, specialty chemical, consumer product and agricultural sectors. However, protein stabilization is highly dependent on the solvent environment and, hence, it is very complex to tailor protein-solvent combinations for stable protein maintenance. Understanding solvent features that govern protein stabilization will enable selection or design of suitable media with favourable solution environments to retain protein native conformation. In this work the structural conformation and activity of lysozyme in 29 solvent systems were investigated to determine the role of various solvent f..View full abstract
E. C. W. is the recipient of a University of Melbourne International Fee Remission Award, CSIRO Materials Science and Engineering PhD Studentship and Albert Shimmins Writing Up Award. This research was undertaken in part on the SAXS/WAXS beamline at the Australian Synchrotron, Melbourne, Australia. The authors are grateful for the help and advice from Dr Nigel Kirby, SAXS/WAXS beamline scientist at the Australian Synchrotron, as well as Prof. Ewan Blanch for his help in FTIR data interpretation.