Journal article
Interaction of the antimicrobial peptides caerin 1.1 and aurein 1.2 with intact bacteria by 2H solid-state NMR
M Laadhari, AA Arnold, AE Gravel, F Separovic, I Marcotte
Biochimica Et Biophysica Acta Biomembranes | ELSEVIER SCIENCE BV | Published : 2016
Abstract
Nuclear magnetic resonance (NMR) is commonly used to probe the effect of antimicrobial agents on bacterial membranes using model membrane systems. Ideally, considering the complexity of membranes, the interaction of molecules with membranes should be studied in vivo. The interactions of two antimicrobial peptides (AMPs) with intact Escherichia coli and Bacillus subtilis were investigated using deuterium solid-state NMR. Specifically, we studied caerin 1.1 and aurein 1.2 isolated from the skin of Australian tree frogs. The minimal inhibitory concentration value for E. coli and B. subtilis was about 100 μg/mL and 30 μg/mL, respectively, for both peptides. A protocol to deuterate the membrane p..
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Awarded by Australian Research Council
Funding Acknowledgements
This work was supported by the Natural Sciences and Engineering Research Council of Canada (NSERC, grant 326750-2013), and the Australian Research Council (grant DP140102127). For the award of scholarships, M.L. is grateful to the Groupe de Recherche Axe sur la Structure des Proteines (GRASP), to Pharmaqam and to the Faculte des Sciences of the Universite du Quebec a Montreal, and A.E.G. to the Canadian Institutes of Health Research Chemical Biology training program and the NSERC of Canada. The authors would like to thank E. Deziel and F. Jean-Pierre (INRS-IAF), and M. Babin (UQAR) for technical advice and support, as well as D.E. Warschawski for stimulating discussions. I.M. is a member of the GRASP and the Centre de recherche sur les Materiaux Fonctionnels.