Journal article

V-1-LIKE [ARG(8)]VASOPRESSIN BINDING-SITES OCCUR IN RAT HEPATOCYTE NUCLEI

PA PHILLIPS, AM HUTCHINS, LM BURRELL, J RISVANIS, CI JOHNSTON

European Journal of Pharmacology | ELSEVIER | Published : 1994

Abstract

Arginine vasopressin binding site characterisation was performed on purified nuclei and plasma membranes from livers of Sprague-Dawley rats. [125I][d(CH2)5,Sarc7,Arg8]vasopressin, a selective V1 vasopressin receptor antagonist radioligand, bound to the nuclei in a protein concentration and time dependent manner. Scatchard analysis of nuclear binding sites revealed a single binding site with maximal binding site density (Bmax) of 115 +/- 13 fmol/mg protein and affinity (KD) of 5.2 +/- 0.7 nM. Plasma membrane binding demonstrated a Bmax of 529 +/- 25 fmol/mg protein and KD of 1.9 +/- 0.1 nM. The displacement profile for nuclear binding sites using vasopressin analogues was similar to that for ..

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