Microtiter enzyme-linked immunosorbent assay for immunoglobulin G cholera antitoxin in humans: Sensitivity and specificity

Abstract

Serum samples were obtained from 92 informed, community volunteers before and 10, 21, and 28 days after they ingested 103 to 106 Vibrio cholerae of Inaba or Ogawa serotype and classical or El Tor biotype as part of a cholera vaccine development program. Pre- and postchallenge sera were examined for neutralizing antibody to cholera toxin by the rabbit skin permeability factor and adrenal cell techniques. Immunoglobulin G-binding antibodies to cholera toxin were quantitated by enzyme-linked immunosorbent assay (ELISA) in serum diluted 1:200. The results obtained in these cholera volunteers were compared with a negative control population comprising 30 people who ingested enteropathogenic Esche..