Journal article
Novel bilayer bacterial nanocellulose scaffold supports neocartilage formation invitro and invivo
H Martínez Ávila, EM Feldmann, MM Pleumeekers, L Nimeskern, W Kuo, WC de Jong, S Schwarz, R Müller, J Hendriks, N Rotter, GJVM van Osch, KS Stok, P Gatenholm
Biomaterials | ELSEVIER SCI LTD | Published : 2015
Abstract
Tissue engineering provides a promising alternative therapy to the complex surgical reconstruction of auricular cartilage by using ear-shaped autologous costal cartilage. Bacterial nanocellulose (BNC) is proposed as a promising scaffold material for auricular cartilage reconstruction, as it exhibits excellent biocompatibility and secures tissue integration. Thus, this study evaluates a novel bilayer BNC scaffold for auricular cartilage tissue engineering. Bilayer BNC scaffolds, composed of a dense nanocellulose layer joined with a macroporous composite layer of nanocellulose and alginate, were seeded with human nasoseptal chondrocytes (NC) and cultured invitro for up to 6 weeks. To scale up ..
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Awarded by Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
Funding Acknowledgements
This study was performed within the framework of EuroNanoMed (EAREG-406340-131009/1) and was supported by the Swedish Research Council (2009-7838), Federal Ministry of Education and Research (13N11076), SenterNovem (ENM09001) and the Swiss National Science Foundation (NRP63). The authors acknowledge Dr. Annette Jork and Cell Med AG (Alzenau, Germany) for providing medical grade alginate; Athanasios Mantas and Anders Martensson at Chalmers University of Technology (Gothenburg, Sweden) for assistance with scaffold production and FTIR analysis, respectively; and Priscila Martinez Avila at The University of Texas at Arlington for helping with statistical analysis. The antibody used for immunohistochemical type II collagen detection (II-II6B3) was obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at the University of Iowa, Department of Biology, Iowa City, IA 52242, USA.