Journal article
Bioengineered 3D platform to explore cell-ECM interactions and drug resistance of epithelial ovarian cancer cells
D Loessner, KS Stok, MP Lutolf, DW Hutmacher, JA Clements, SC Rizzi
Biomaterials | ELSEVIER SCI LTD | Published : 2010
Abstract
The behaviour of cells cultured within three-dimensional (3D) structures rather than onto two-dimensional (2D) culture plastic more closely reflects their in vivo responses. Consequently, 3D culture systems are becoming crucial scientific tools in cancer cell research. We used a novel 3D culture concept to assess cell-matrix interactions implicated in carcinogenesis: a synthetic hydrogel matrix equipped with key biomimetic features, namely incorporated cell integrin-binding motifs (e.g. RGD peptides) and the ability of being degraded by cell-secreted proteases (e.g. matrix metalloproteases). As a cell model, we chose epithelial ovarian cancer, an aggressive disease typically diagnosed at an ..
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Funding Acknowledgements
The authors are grateful to Dr Deborah Stenzel, Dr Christina Theodoropoulos and Dr Luke Nothdurft from the Analytical Electron Microscopy Facility of the Queensland University of Technology (QUT) and Dr Leonore de Boer from the Cell Imaging Facility of the Institute of Health and Biomedical Innovation (IHBI) for their assistance in the microscopy techniques. This study was supported by the National Health and Medical Research Council (NHMRC) of Australia, a Smart State Fellowship of the Queensland Government, QUT and Tissue Therapies Ltd. (Brisbane, Australia) and two Early Career Research Awards, IHBI, QUT, Australia.