Journal article

Simplified Murine 3D Neuronal Cultures for Investigating Neuronal Activity and Neurodegeneration

Steven J Collins, Cathryn L Haigh

CELL BIOCHEMISTRY AND BIOPHYSICS | HUMANA PRESS INC | Published : 2017

Abstract

The ability to model brain tissue in three-dimensions offers new potential for elucidating functional cellular interactions and corruption of such functions during pathogenesis. Many protocols now exist for growing neurones in three-dimensions and these vary in complexity and cost. Herein, we describe a straight-forward method for generating three-dimensional, terminally differentiated central nervous system cultures from adult murine neural stem cells. The protocol requires no specialist equipment, is not labour intensive or expensive and produces mature cultures within 10 days that can survive beyond a month. Populations of functional glutamatergic neurones could be identified within cultu..

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Funding Acknowledgements

This work was funded by an NHMRC programme grant (SJC, #628946) and an NHMRC project grant (CLH, #APP1044264). SJC is supported by an NHMRC Practitioner Fellowship (#APP100581) and CLH is supported by a CJDSGN Memorial Grant in memory of Rhonda McCoy and the many people affected by prion disease in Australia.