The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development

Antanas Spokevicius; Lynette Taylor; Emma Melder; Kim Van Beveren; Josquin Tibbits; Nicky Creux; Gerd Bossinger

Journal article

The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development

Antanas Spokevicius, Lynette Taylor, Emma Melder, Kim Van Beveren, Josquin Tibbits, Nicky Creux, Gerd Bossinger

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | JOURNAL OF VISUALIZED EXPERIMENTS | Published : 2016

DOI: 10.3791/54553

Abstract

Secondary stem growth in trees and associated wood formation are significant both from biological and commercial perspectives. However, relatively little is known about the molecular control that governs their development. This is in part due to physical, resource and time limitations often associated with the study of secondary growth processes. A number of in vitro techniques have been used involving either plant part or whole plant system in both woody and non-woody plant species. However, questions about their applicability for the study of secondary stem growth processes, the recalcitrance of certain species and labor intensity are often prohibitive for medium to high throughput applica..

View full abstract

University of Melbourne Researchers

Antanas Spokevicius Author

Gerd Bossinger Author

Related Projects (3)

Functional genetic analysis of wood formation genes in Eucalyptus

Eucalypts are the world's most important plantation hardwood species due to their superior wood characteristics and high growth rates that m..

Functional genetic analysis of wood formation genes in Eucalyptus

Eucalypts are the world's most important plantation hardwood species due to their superior wood characteristics and high growth rates that m..

IDENTIFICATION OF GENES CONTROLLING WOOD FIBRE PROPERTIES IN EUCALYPTUS NITEN

Australian forestry is shifting to high intensity eucalypt plantations and increasingly focussing on development of breeds and clones with s..

Grants

Awarded by Linkage Grants

Funding Acknowledgements

The authors would like to acknowledge funding support for aspect of the work through Linkage Grants LP0776563 (GB, AS) and LP0211919 (GB) and industry partners Sappi and Mondi as well as Australian Postgraduate Award (EM) from the Australian Research Council and the Young Innovators and Scientist Award through the Australian Department of Agriculture (LT). We also like to thank the Zander Myburg, Qing Wang, Colleen MacMillan and Simon Southerton for the many discussions and ideas they put forward during the development of this protocol and to Martin Ranik, Minique De Castro, Julio Najera, Valerie Frassiant, Angelique Manuel and Noemie Defaix for assistance in laboratory related work.