A Novel Role for SIRT3 in Regulating Mediators Involved in the Terminal Pathways of Human Labor and Delivery
Ratana Lim, Gillian Barker, Ramkumar Menon, Martha Lappas
BIOLOGY OF REPRODUCTION | OXFORD UNIV PRESS INC | Published : 2016
Preterm birth remains the major cause of neonatal mortality and morbidity, mediated largely by an inflammatory process. The sirtuin (SIRT) family of cellular regulators has been implicated as key inhibitors of inflammation. We have previously reported a role for SIRT1, SIRT2, and SIRT6 in regulating inflammation-induced prolabor mediators. In this study, we determined the effect of term labor and pro-inflammatory cytokines on SIRT3, SIRT4, SIRT5, and SIRT7 expression in human myometrium. Functional studies were also used to investigate the effect of small interfering RNA (siRNA) knockdown of SIRTs in regulating inflammation-induced prolabor mediators. Western blot analysis and qRT-PCR were u..View full abstract
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Awarded by National Health and Medical Research Council (NHMRC) Career Development Fellowship
Awarded by NHMRC
Supported by National Health and Medical Research Council (NHMRC) Career Development Fellowship 1047025 to M.L, and NHMRC grant no. 1058786, the Norman Beischer Medical Research Foundation, and the Mercy Research Foundation.