Journal article

High concordance between Illumina HiSeq2500 and NextSeq500 for reduced representation bisulfite sequencing (RRBS)

Danqing Yin, Matthew E Ritchie, Jafar S Jabbari, Tamara Beck, Marnie E Blewitt, Andrew Keniry

GENOMICS DATA | ELSEVIER SCIENCE BV | Published : 2016

Abstract

Reduced representation bisulfite sequencing (RRBS) provides an efficient method for measuring DNA methylation at single base resolution in regions of high CpG density. This technique has been extensively tested on the HiSeq2500, which uses a 4-colour detection method, however it is unclear if the method will also work on the NextSeq500 platform, which employs a 2-colour detection system. We created an RRBS library and sequenced it on both the HiSeq2500 and NextSeq500, and found no significant difference in the base composition of reads derived from either machine. Moreover, the methylation calls made from the data of each instrument were highly concordant, with methylation patterns across th..

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Grants

Awarded by Australian National Health and Medical Research Council


Funding Acknowledgements

We thank The Dyson Bequest and The DHB Foundation for philanthropic funding to MEB. This work was supported by grants from the Australian National Health and Medical Research Council (GNT1045936) to MEB. MEB and MER are RD Wright Biomedical Research fellows of the Australian National Health and Medical Research Council (GNT1110206 and GNT1104924 respectively). We thank the AGRF for producing the RRBS library and sequencing on the HiSeq2500 platform, and Dr. Stephen Wilcox (WEHI) for sequencing on the Nextseq500. This work was made possible through Victorian State Government Operational Infrastructure Support and Australian National Health and Medical Research Council Research Institute Infrastructure Support Scheme.