Journal article
Cycloheximide can induce bax/bak dependent myeloid cell death independently of multiple BH3-only proteins
KJ Goodall, ML Finch-Edmondson, J Van Vuuren, GC Yeoh, IE Gentle, JE Vince, PG Ekert, DL Vaux, BA Callus
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2016
Abstract
Apoptosis mediated by Bax or Bak is usually thought to be triggered by BH3-only members of the Bcl-2 protein family. BH3-only proteins can directly bind to and activate Bax or Bak, or indirectly activate them by binding to anti-apoptotic Bcl-2 family members, thereby relieving their inhibition of Bax and Bak. Here we describe a third way of activation of Bax/Bak dependent apoptosis that does not require triggering by multiple BH3-only proteins. In factor dependent myeloid (FDM) cell lines, cycloheximide induced apoptosis by a Bax/Bak dependent mechanism, because Bax-/- Bak-/- lines were profoundly resistant, whereas FDM lines lacking one or more genes for BH3-only proteins remained highly se..
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Awarded by National Science Foundation
Funding Acknowledgements
[ "This work was supported by an Association of International Cancer Research project grant (09-0084) and Cancer Council of WA project grants (1010749 and 1067515) and fellowship to BAC, and National Health and Medical Research Council (Canberra, Australia) Project grant (384404), fellowship (1016701), and Program Grant (461221) to DLV, and operational infrastructure grants through the Australian Government IRISS and the Victorian State Government OIS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.", "This work was supported by an Association of International Cancer Research project grant (09-0084) and Cancer Council of WA project grants (1010749 and 1067515) and fellowship to BAC, and National Health and Medical Research Council (Canberra, Australia) Project grant (384404), fellowship (1016701), and Program Grant (461221) to DLV, and operational infrastructure grants through the Australian Government IRISS and the Victorian State Government OIS. We generously thank Andreas Strasser for providing Bcl-2 antibody, Lorraine O'Reilly for providing Bcl-w and Bcl-xL antibodies, David Huang for providing A1 antibody and Mcl-1 and Bcl-2 cDNAs and Doug Fairlie for providing BimS2A cDNA." ]