Oral swirl samples - a robust source of microRNA protected by extracellular vesicles
T Yap, LJ Vella, C Seers, A Nastri, E Reynolds, N Cirillo, M McCullough
ORAL DISEASES | WILEY | Published : 2017
BACKGROUND: MicroRNAs are small non-coding RNAs which are dysregulated in disease states, such as oral cancer. Extracellular vesicles, a potential source of microRNA, are found in saliva. OBJECTIVE: To demonstrate that a quantifiable amount of microRNA can be isolated from oral swirl samples. Additionally, we hypothesized that extracellular vesicles may protect contained microRNA from degradation in these samples. METHOD: A polyethylene glycol-based precipitation was used for extracellular vesicle enrichment of oral swirl samples. Comparison was made between samples treated with and without RNase. Further, samples from three subjects were exposed to a range of conditions over 7 days and asse..View full abstract
This work was supported by the Australian Dental Research Foundation, the Australia & New Zealand Head and Neck Cancer Society Research Foundation and the Oral Health Cooperative Research Centre.