Journal article

Oral swirl samples - a robust source of microRNA protected by extracellular vesicles

T Yap, LJ Vella, C Seers, A Nastri, E Reynolds, N Cirillo, M McCullough

ORAL DISEASES | WILEY | Published : 2017

Abstract

BACKGROUND: MicroRNAs are small non-coding RNAs which are dysregulated in disease states, such as oral cancer. Extracellular vesicles, a potential source of microRNA, are found in saliva. OBJECTIVE: To demonstrate that a quantifiable amount of microRNA can be isolated from oral swirl samples. Additionally, we hypothesized that extracellular vesicles may protect contained microRNA from degradation in these samples. METHOD: A polyethylene glycol-based precipitation was used for extracellular vesicle enrichment of oral swirl samples. Comparison was made between samples treated with and without RNase. Further, samples from three subjects were exposed to a range of conditions over 7 days and asse..

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