Journal article

RELATION OF VP7 AMINO-ACID-SEQUENCE TO MONOCLONAL-ANTIBODY NEUTRALIZATION OF ROTAVIRUS AND ROTAVIRUS MONOTYPE

BS COULSON, C KIRKWOOD

Journal of Virology | AMER SOC MICROBIOLOGY | Published : 1991

Abstract

The neutralization epitopes of the VP7 of human rotavirus RV-4 were studied by using five neutralizing mouse monoclonal antibodies to select virus variants resistant to neutralization by each of the antibodies. Antibody resistance patterns and sequence analysis of the RV-4 variants revealed that at least four sites on VP7, located at amino acids 94 (region A), 147 to 148 (region B), 213 (region C), and 291, are involved in neutralization of the human G1 rotavirus RV-4. The A-region site elicited antibody cross-reactive between G types and showed species-restricted immunodominance not related to carbohydrate attachment. The monotype 1b rotavirus M37 lacked this site. The B region contained st..

View full abstract

University of Melbourne Researchers