Journal article

COMPARATIVE SENSITIVITIES OF SOLID-PHASE IMMUNE ELECTRON-MICROSCOPY AND ENZYME-LINKED IMMUNOSORBENT-ASSAY FOR SEROTYPING OF HUMAN ROTAVIRUS STRAINS WITH NEUTRALIZING MONOCLONAL-ANTIBODIES

G GERNA, A SARASINI, BS COULSON, M PAREA, M TORSELLINI, E ARBUSTINI, M BATTAGLIA

Journal of Clinical Microbiology | AMER SOC MICROBIOLOGY | Published : 1988

Abstract

Suspensions of 24 rotavirus strains, 6 for each known human rotavirus serotype, were serially diluted and titrated by (i) enzyme-linked immunosorbent assay (ELISA) for rotavirus detection, using monoclonal antibodies (MAbs) specific for group-specific sites of the VP6 inner capsid protein; (ii) ELISA for subgrouping, using MAbs reactive with subgroup-specific determinants of rotavirus VP6; (iii) ELISA for serotyping, using MAbs directed to serotype-specific sites of the VP7 outer capsid glycoprotein; and (iv) solid-phase immune electron microscopy (SPIEM) for serotyping, using VP7-specific MAbs. In addition, in each preparation the proportion of double-shelled rotavirus particles were determ..

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