Journal article
Pharmacological characterisation of 5-HT receptors positively coupled to adenylyl cyclase in the rat hippocampus
R Markstein, M Matsumoto, C Kohler, H Togashi, M Yoshioka, D Hoyer
Naunyn Schmiedeberg S Archives of Pharmacology | SPRINGER VERLAG | Published : 1999
DOI: 10.1007/PL00005375
Abstract
The pharmacological properties of 5-hydroxytryptamine (5-HT) receptors positively coupled to adenylyl cyclase in the rat hippocampus were investigated using selective agonists and antagonists. 5-HT (0.008-125 μM) stimulated cyclic AMP formation in homogenates of rat hippocampus in a concentration-dependent manner. The maximal increase in cyclic AMP formation occurred at 1 μM (141 ± 6%) and the half-maximal effect (EC50) at 50 ± 22 nM. Cyclic AMP accumulation induced by 1 μM 5-HT was partly inhibited by the selective 5-HT(1A) receptor antagonist WAY 100,635 (1 μM), the selective 5-HT4 receptor antagonist SB 203,186 (1 μM), and the 5-HT(2A/C)/5-HT7 receptor antagonist mesulergine (25 μM). WAY ..
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